Bot Detector
Download FREE Sample Issue or Article
Subscribe Today
A subscription to IJPC includes a print copy delivered by postal mail and on-line access to electronic PDF copies of your subscribed issues.

Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops

Author(s):  Sahyoun Jean-Yves, Cloutier Marc, Frenette Mathieu, Robert Marie-Claude

Issue:  Jul/Aug 2022 - Volume 26, Number 4
View All Articles in Issue

Abstract:  The objective of this experimental study was to investigate the stability of functional proteins in human serum eye drops, which are used for the treatment of ocular surface disorders, after prolonged storage of 6 months at -20°C. After obtaining whole blood from 3 volunteers and preparing 100% (S100), 50% (S50), and 20% (S20) serum eye drops, fibronectin was quantified before and after storage for 6 months at -20°C using appropriate enzyme-linked immunoassay kits. The pH and microbial contamination of preparations were also evaluated longitudinally. The fibronectin concentration showed no significant reduction in undiluted (S100) or diluted (S50 and S20) serum after 6 months of frozen storage at -20°C. None of the preparations showed any microbial contamination and no significant changes in pH were noted during storage. Frozen serum eye drops appear stable after prolonged storage at -20°C. Fibronectin in serum is temperature and time resistant after prolonged storage at -20°C. While the impact of individual serum proteins on ocular surface health remains unclear, our results suggest that freezing up to 6 months provides adequate preservation of epitheliotropic factors and a minimal risk of microbial contamination.

Related Keywords: Jean-Yves Sahyoun, MD, Marc Cloutier, PhD, Mathieu Frenette, PhD, Marie-Claude Robert, MD, MSc, FRCSC, human allogeneic serum eye drops, functional proteins, ocular surface disorders, eye disorders, fibronectin, microbial contamination, physical stability, chemical stability, microbiological stability, cytokines, growth factors, vitamins, human tears, wound healing, freezing


Printer-Friendly Version

Download in electronic PDF format for $65

Related Articles from IJPC
Title (Click for Abstract / Details) Author Issue Page View/Buy
Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Sahyoun Jean-Yves, Cloutier Marc, Frenette Mathieu, Robert Marie-Claude Jul/Aug 2022 336-341 Buy
Stability and In Vitro Toxicity of an Infliximab Eye Drop Formulation Robert Marie-Claude, Spurr-Michaud Sandra, Frenette Mathieu, Young David, Gipson Ilene K, Dohlman Claes H Sep/Oct 2014 418-426 Buy
Protocol of Blood Serum Eye Drops Katsakoulas Ioannis, Lougovoi Claudia, Paraskevopoulou Penelope, Vougioukas Nikolaos May/Jun 2015 252-260 Buy
Physical and Chemical Stability of Pharmaceutical Preparation of Bumetanide and Scopolamine Catry Emilie, Colsoul Marie-Louise, Closset Melanie, Hubert Justine, Soumoy Laura, Bihin Benoit, Thiry Emmanuell, Jamart Jacques, Hecq Jean-Daniel, Galanti Laurence May/Jun 2022 248-254 Buy
Autologous Eye Drops for the Treatment of Dry Eye and Neurotrophic Keratitis Mixon William, Angelle Patricea (Patsy), Chang Richard I Nov/Dec 2009 506-515 Buy
Quality-Control Analytical Methods: Applications and Sterility of Autologous Eye Drops Reed-Kane Dana, Carlson Rachel A, Kupiec Thomas C, Vu Nicole Nov/Dec 2009 540-543 Buy
Autologous Serum Eye Drops for Severe Dry Eye Syndrome in a Patient with Chronic Graft-vs-Host Disease: A Case Report Mixon Bill, Mixon Jan, Isbey Edward K III, Sprinkle Shari Sep/Oct 2014 370-377 Buy
Measurement of Free Iodine in Different Formulations of Povidone-Iodine Eye Drops 5% Berisa Prado Silvia, Riestra Ayora Ana Cristina, Trelles Campa Adrián Sep/Oct 2019 418-421 Buy
Physical and Chemical Stability of Urapidil in 0.9% Sodium Chloride in Elastomeric Infusion Pump Tomasello Cristina, Leggieri Anna, Rabbia Franco, Veglio Franco, Baietto Lorena, Fulcheri Chiara, De Nicolò Amedeo, De Perri Giovanni, D'Avolio Antonio Jul/Aug 2016 343-346 Buy
Long-term Physicochemical Stability of Concentrated Solutions of Sodium Valproate in Polypropylene Syringes for Administration in the Intensive Care Unit Lardinois Benjamin, Baltzis Alexandre, Braibant Maximilien, Soumoy Laura, Jamart Jacques, Bihin Benoît, Hecq Jean-Daniel, Galanti Laurence Jul/Aug 2019 320-323 Buy
Particle Measuring Systems - USP <797> Environmental Monitoring Solutions