Abstract

Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops

Author(s): Sahyoun Jean-Yves, Cloutier Marc, Frenette Mathieu, Robert Marie-Claude

Issue: Jul/Aug 2022 - Volume 26, Number 4

Page(s): 336-341

Download in electronic PDF format for $75
  • Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Page 1
  • Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Page 2
  • Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Page 3
  • Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Page 4
  • Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Page 5
  • Long-term Stability of Epitheliotropic Factors in Frozen Serum Eye Drops Page 6

Abstract

The objective of this experimental study was to investigate the stability of functional proteins in human serum eye drops, which are used for the treatment of ocular surface disorders, after prolonged storage of 6 months at -20°C. After obtaining whole blood from 3 volunteers and preparing 100% (S100), 50% (S50), and 20% (S20) serum eye drops, fibronectin was quantified before and after storage for 6 months at -20°C using appropriate enzyme-linked immunoassay kits. The pH and microbial contamination of preparations were also evaluated longitudinally. The fibronectin concentration showed no significant reduction in undiluted (S100) or diluted (S50 and S20) serum after 6 months of frozen storage at -20°C. None of the preparations showed any microbial contamination and no significant changes in pH were noted during storage. Frozen serum eye drops appear stable after prolonged storage at -20°C. Fibronectin in serum is temperature and time resistant after prolonged storage at -20°C. While the impact of individual serum proteins on ocular surface health remains unclear, our results suggest that freezing up to 6 months provides adequate preservation of epitheliotropic factors and a minimal risk of microbial contamination.

Related Keywords

Related Categories

Printer-Friendly Version

Related Articles from IJPC

Issue/Page
View/Buy
Title/Author
(Click for Abstract / Details / Purchase)
Jul/Aug 2022
Pg. 336-341
May/Jun 2015
Pg. 252-260
Sep/Oct 2014
Pg. 418-426
Nov/Dec 2009
Pg. 506-515
Nov/Dec 2009
Pg. 540-543
Sep/Oct 2014
Pg. 370-377
Sep/Oct 2019
Pg. 418-421
Jul/Aug 2017
Pg. 282-289
Author(s): Choo Winnie
May/Jun 2018
Pg. 240-246
Sep/Oct 2008
Pg. 456-459
Mar/Apr 2004
Pg. 156-159
Jul/Aug 2022
Pg. 330-335
Nov/Dec 2022
Pg. 446-466
Author(s): Allen Loyd V Jr
Sep/Oct 2014
Pg. 427-431
Jul/Aug 2016
Pg. 347-350
Jul/Aug 2017
Pg. 319
Author(s): Allen Loyd V Jr
Jan/Feb 2004
Pg. 46-48
Author(s): Allen Loyd V Jr
May/Jun 2017
Pg. 223-228
Author(s): Akers Michael J
Jul/Aug 2017
Pg. 322-329
Jul/Aug 1997
Pg. 253
Author(s): Allen Loyd V Jr