Logo - International Journal of Pharmaceutical Compounding

Quantitation of Testosterone in Suspension using High Performance Liquid Chromatography

Author(s):  Gupta Vishnu D

Issue:  May/Jun 1999 - Compounding for Animals
View All Articles in Issue

Abstract:  An assay method based on high-performance liquid chromatography (HPLC), for the quantitation of testosterone in an aqueous suspension, has been developed. The method is very simple compared with a tedious and time-consuming method recommended by the USP-NF. It is an accurate and precise method; the percent relative standard deviation based on five injections was 1.15. The average recovery from four batches of suspension was 100% (percent relative standard deviation, 1.3). A number of inactive ingredients present in the suspension vehicle did not interfere with the assay procedure. Testosterone appears to be a very stable drug since it did not decompose significantly, even on heating. A 15-cm column gave sharper peaks vs. a 25-cm column and the sensitivity of the assay method increased by 400%.

Related Keywords: high performance liquid chromatography, HPLC, aqueous suspension


Purchase this article for download in electronic PDF format from IJPC at for $35 at:

Search the entire IJPC archive by keyword, topic, or issue at: