Quantitation of Testosterone in Suspension using High Performance Liquid Chromatography
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Abstract: An assay method based on high-performance liquid chromatography
(HPLC), for the quantitation of testosterone in
an aqueous suspension, has been developed. The method is very
simple compared with a tedious and time-consuming method
recommended by the USP-NF. It is an accurate and precise
method; the percent relative standard deviation based on five
injections was 1.15. The average recovery from four batches
of suspension was 100% (percent relative standard deviation,
1.3). A number of inactive ingredients present in the suspension
vehicle did not interfere with the assay procedure. Testosterone
appears to be a very stable drug since it did not decompose
significantly, even on heating. A 15-cm column gave sharper
peaks vs. a 25-cm column and the sensitivity of the assay
method increased by 400%.
Related Keywords:
high performance liquid chromatography, HPLC, aqueous suspension
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