Bot Detector
Download FREE Sample Issue or Article
Subscribe Today
A subscription to IJPC includes a print copy delivered by postal mail and on-line access to electronic PDF copies of your subscribed issues.

Stability of Ampicillin in Normal Saline and Buffered Normal Saline

Author(s):  Maher Moureddine, Jensen Kara J, Lee David, Nix David E

Issue:  Jul/Aug 2016 - Volume 20, Number 4
View All Articles in Issue

Abstract:  Ampicillin is reported to be stable for only 8 hours in 0.9% sodium chloride with concentrations up to 30 g/L. The purpose of this study was to evaluate if the stability of ampicillin in 0.9% sodium chloride could be extended by adding sodium phosphate for injection using modern highperformance liquid chromatographic methods. Ampicillin was prepared at concentrations of 12 g/L with and without 10 mM sodium phosphate for injection. Stability was assessed under room temperature using a validated, stability- indicating high-performance liquid chromatography assay. The admixtures with added buffer were studied under refrigerated storage as well. Ampicillin 12 g in 1 L normal saline intravenous bags, without buffer addition, was stable for at least 24 hours when stored at room temperature. The time to 90% potency (T90) ranged from 32.0 hours to 41.7 hours. The addition of 10 mM sodium phosphate buffer extended the stability to at least 48 hours with a T90 of 57.6 hours. Ampicillin 12 g/L stored under refrigeration was stable for at least 72 hours. Considering cumulative loss during refrigerated storage and subsequent use at room temperature, the data supports up to 48 hours storage under refrigeration and then up to 24 hours during use at room temperature. Using a stabilityindicating high-performance liquid chromatographic assay, ampicillin 12 g/L in 0.9% sodium chloride is stable for at least 24 hours at room temperature. The addition of 10 mM sodium phosphate for injection extends the stability to at least 48 hours at room temperature.

Related Keywords: Noureddine Maher, MS, Kara J. Jensen, David Lee, BS (Pharmacy), David E. Nix, PharmD, BCPS, AQ-ID, ampicillin, antibiotic, antimicrobial, enterococcus, enterococcal infection, stability, sodium phosphate


Printer-Friendly Version

Download in electronic PDF format for $65

Related Articles from IJPC
Title (Click for Abstract / Details) Author Issue Page View/Buy
Stability of Ampicillin in Normal Saline and Buffered Normal Saline Maher Moureddine, Jensen Kara J, Lee David, Nix David E Jul/Aug 2016 338-342 Buy
Stability of Ampicillin Sodium, Nafcillin Sodium, and Oxacillin Sodium in AutoDose Infusion System Bags Zhang Yanping, Trissel Lawrence A May/Jun 2002 226-229 Buy
Palonosetron Hydrochloride Compatibility and Stability with Three Beta-Lactam Antibiotics During Simulated Y-Site Administration Ben Michel, Trusley Craig, Kupiec Thomas C, Trissel Lawrence A Nov/Dec 2007 520-524 Buy
Stability of Extemporaneously Prepared Sodium Benzoate Oral Suspension Atkins Julia R, Lull Melinda E, Decker Andrew S, Hutchinson David J Jul/Aug 2018 326-328 Buy
Development and Validation of a Stability-indicating High-performance Liquid Chromatographic Method for Quantification of Progesterone in Compounded Glycerinated Gelatin Troches Burrows Anna C, Yellepeddi Venkata Kashyap, Snyder Spencer, Wakefield Meagan, Jackson David, Huynh Johnny, Nguyen Khoa, Sayre Casey L Jul/Aug 2019 340-350 Buy
Physicochemical and Microbiological Stability of Vancomycin 10-mg/mL Intravitreal Syringes Bartoll Aaron Puplà, Bellés Medall Maria Dolores, Peñarrocha Josep Edo, Escrig Laura Solaz, Clemente Ruben Eguren, Azuara Julia Bodega, Piqueres Raul Ferrando Jul/Aug 2022 330-335 Buy
Certification, Accreditation, and Credentialing for 503A Compounding Pharmacies Pritchett Jon, McCrory Gary, Kraemer Cheri, Jensen Brenda, Allen Loyd V Jr Jan/Feb 2018 7-16 Buy
Comparative Stability of Vitamin K1 Oral Liquids Prepared in Sterile Water for Injection and Stored in Amber Glass Bottles and Amber Plastic Syringes Huffman Jessica, Brown Stacy, Lewis Paul, Lawson Sarah, Ogle Amanda, Peacock Gina Jul/Aug 2018 340-344 Buy
Finding the Right Balance: A Nationwide Study of Student Pharmacist Exposure to and Perceptions of Nonsterile Compounding Fredrickson Mary E, Belcher Faith G, Del Vecchio Kamryn N, Galleher Miranda N, Jugo Leila S, Gothard M David, Pallotta Raneem H, Pallotta Victor A Jr May/Jun 2022 242-247 Buy
Descriptive Report of Individual State Responses to Personal Protective Equipment Shortages for Pharmacy Practice During the Covid-10 Pandemic Roberts Patricia A, Eberwein Samuel M, Youmans Danielle, Amerine Lindsey B Mar/Apr 2021 109-113 Buy
Particle Measuring Systems - USP <797> Environmental Monitoring Solutions