Quality-Control Analytical Methods: High-Performance Liquid Chromatography
Author(s): Kupiec Thomas C
Issue: May/Jun 2004 - Andropause
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Page(s): 223-227
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Abstract: Chromatography is an analytical technique based on the separation of molecules due to differences in their structure and/or composition. In this article, the author discusses practical aspects of high-performance liquid chromatography theory (chromatographic principles such as retention, resolution and sensitivity), chromatographic mechanisms, instrumentation, mobile phase and reservoir, pumps, injectors, detectors, data-acquisition/display systems and columns, separation techniques (isocratic vs. gradient elution and derivatization), quantitative analysis (external standard and internal standard) and validation. Figures show a chromatogram of a reference standard and a sample. High-performance liquid chromatography is currently the most widely used method of quantitative analysis in the pharmaceutical industry and in pharmaceutical analytical laboratories.
Related Keywords: High-performance liquid chromatography, quality-control analytical methods for, HPLC, quality-control analytical methods for, Kupiec, Tom, PhD, Quality-control analytical methods, for high-performance liquid chromatography