Bot Detector
IJPC Seal
Download FREE Sample Issue or Article
LEARN MORE
Subscribe Today
A subscription to IJPC provides on-line access to full-text, full-color, printable PDF copies of all issues and individual articles published by the Journal since 1997.

Quantitation of Testosterone Propionate in an Injection Using High Performance Liquid Chromatography

Author(s):  Gupta Vishnu D

Issue:  Mar/Apr 1999 - Compounding for Men's Health
View All Articles in Issue

Page(s):  158-159

Quantitation of Testosterone Propionate in an Injection Using High Performance Liquid Chromatography Page 1
Quantitation of Testosterone Propionate in an Injection Using High Performance Liquid Chromatography Page 2

Download in electronic PDF format for $75

Abstract:  A high-performance liquid chromatography assay method for the quantitation of testosterone propionate in an injection has been developed. The assay method is very simple, precise and accurate, with a percent relative standard deviation of 1.3 based on five injections. The benzyl alcohol (the preservative) and sesame oil (the vehicle) did not interfere with the assay procedure. The extraction procedure is very simple compared with a tedious and time-consuming method recommended in the USP-NF. The other similar compounds, testosterone, methyltestosterone, testosterone cypionate and testosterone enanthate, did not interfere with the assay method. Hence, the method developed is specific for this drug, versus the USP-NF method, which is nonspecific.

Related Keywords: high-performance liquid chromatography, HPLC, methyltestosterone, testosterone cypionate

Related Categories: HRT, PEER-REVIEWED

Printer-Friendly Version



Related Articles from IJPC
Title/Author
(Click for Abstract / Details / Purchase)
Issue/​Page
View/Buy
Quantitation of Testosterone-Cypionate-in-Oil Injection using High Performance Liquid Chromatography
Gupta Vishnu D
, Pramar Y
Jul/Aug 1999
Pg. 314-315

Quantitation of Testosterone Propionate in an Injection Using High Performance Liquid Chromatography
Gupta Vishnu D
Mar/Apr 1999
Pg. 158-159

Quality-Control Analytical Methods: High-Performance Liquid Chromatography
Kupiec Thomas C
May/Jun 2004
Pg. 223-227

Quantitation of Testosterone in Suspension using High Performance Liquid Chromatography
Gupta Vishnu D
May/Jun 1999
Pg. 239-240

Stability of an Oral Liquid Dosage Form of Trovafloxacin Mesylate and Its Quantitation in Tablets Using High-Performance Liquid Chromatography
Gupta Vishnu D
May/Jun 2000
Pg. 233-235

A New High-Performance Liquid Chromatographic Method for Fludarabine and Fludarabine Phosphate Compounded in Liposomes
Abang Anthony M
, Abbas S Ali, Pham Trinh, Lambros Maria Polikandritou
Jul/Aug 2001
Pg. 320-322

Stability of Acetyl-L-carnitine in 5% Dextrose Using a High-Performance Liquid Chromatography–Mass Spectrometry Times 2 Method
Zhang Yang
, Jiang Hongliang, Hutson Paul
Mar/Apr 2012
Pg. 170-173

Development and Application of a High-Performance Liquid Chromatography Stability-Indicating Assay for Beyond-Use Date Determination of Compounded Topical Gels Containing Multiple Active Drugs
Gorman Gregory
, Sokom Simara, Coward Lori, Arnold John J
Mar/Apr 2017
Pg. 164-170

Testosterone Cypionate 100-mg/mL Injection
Allen Loyd V Jr
Jan/Feb 2013
Pg. 73

Quality-Control Analytical Methods: Gas Chromatography
Kupiec Thomas C
Jul/Aug 2004
Pg. 305-309

Using Hospital Laboratory Services to Perform Quality-control Testing on Compounded Preparations
Donnelly Ronald F
Sep/Oct 2015
Pg. 383-386

High-Performance Liquid Chromatographic Method Optimization for Ondansetron Assay in Extemporaneous Topical Gel and in Marketed Products
Quamrun Masuda
, Mamoon Rashid, Nasheed Shams, Mullins Randy
Nov/Dec 2014
Pg. 520-525

Validation of Ultraviolet-visible and High-performance Liquid Chromatographic Methods for the Determination of Sodium p-Aminosalicylate and m-Aminophenol in a New Pharmaceutical Formulation
Hergert Lisandro Y
, Ravetti Soledad, Mazzieri Maria R
Jan/Feb 2016
Pg. 63-70

Development and Validation of a Stability-indicating High-performance Liquid Chromatographic Method for Quantification of Progesterone in Compounded Glycerinated Gelatin Troches
Burrows Anna C
, Yellepeddi Venkata Kashyap, Snyder Spencer, Wakefield Meagan, Jackson David, Huynh Johnny, Nguyen Khoa, Sayre Casey L
Jul/Aug 2019
Pg. 340-350

Simple High-Performance Liquid Chromatographic Method for Simultaneous Determination of Fusidic Acid and Betamethasone Dipropionate in a Cream Formulation
Peh Kok-Khiang
, Tan Yvonne Tze-Fung
May/Jun 2000
Pg. 229-231

Stability of Alatrofloxacin Mesylate in 5% Dextrose Injection and 0.45% Sodium-Chloride Injection
Gupta Vishnu D
, Bailey RE
Jan/Feb 2000
Pg. 66-68

Case Study: Absorption of Testosterone Cream via Scrotal Delivery
Needham Shawn
, Needham Shane
Nov/Dec 2018
Pg. 466-468

Subcutaneous Testosterone Anastrozole Therapy in Men: Rationale, Dosing, and Levels on Therapy
Glaser Rebecca L
, York Anne E
Jul/Aug 2019
Pg. 325-339

Compounded Testosterone Troches to optimize Health and the Testosterone Controversy
Guth Michael AS
May/Jun 2015
Pg. 195-203

Testosterone Enanthate 200-mg/mL Injection
Allen Loyd V Jr
Mar/Apr 2014
Pg. 151

Return to Top